pCL-Eco Retrovirus Packaging Vector

Mouse, Rat

N/A

Ecotropic (MoMuLV) for mouse and rat cells only (not human).
Amphotropic (from 4070A MuLV) for most mammalian cells (but not hamster).
10A1 (MuLV) for most mammalian cells (including hamster).

Retroviral Production (reported in scientific literature (PMID 24760698))

The plasmid DNA contains an ampicillin resistant gene. For large scale production, this plasmid may be amplified on LB plates containing 50 ug/ml ampicillin. The liquid cultures may be grown at 100 ug ampicillin/ml.
For amplification, transform a suitable E. coli host such as HB101 or DH-5alpha and plate on LB plates containing 50-ug/ml ampicillin. For plasmid DNA preparation start culture from a single colony in LB medium containing 100 ug/ml ampicillin

Mouse reactivity reported in scientific literature (PMID: 24760698). This product can be used in human cells to produce virions to infect non-human cells for protein production.

10 ug in 20 ul 1x TE (10 mM Tris, pH 7.5, 1 mM EDTA)

Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Store at -20C. Avoid freeze-thaw cycles.