Propidium Iodide
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31155
Key Product Details
Species
Human
Applications
Flow Cytometry, Microscopy
Product Summary for Propidium Iodide
Propidium Iodide is excluded by viable cells but can penetrate cell membranes of dying or dead cells. Dead cells will take up PI and fluoresce brightly in FL2 off of the blue laser on a standard flow cytometer. PI also may be excited by the UV or Green/Yellow lasers on more advanced flow cytometers and may, therefore, not be optimal for certain multicolor panels. It emission maxima is at 617nm.
Product Specifications
Application Notes
Fluorescent stain for nucleic acids. Cell membrane integrity excludes propidium iodide from staining viable and apoptotic cells. Propidium iodide may be used in flow cytometry to evaluate cell viability when used with other dyes that stain viable cells or cells that are early in the apoptosis process.
Reactivity Notes
Use in Human reported in scientific literature (PMID:33800462).
Scientific Data Images for Propidium Iodide
![Flow Cytometry: Propidium Iodide [NBP2-31155]](https://resources.bio-techne.com/images/products/Propidium-Iodide-Flow-Cytometry-NBP2-31155-img0003.jpg "Flow Cytometry: Propidium Iodide [NBP2-31155]")
Flow Cytometry: Propidium Iodide [NBP2-31155]
Flow Cytometry: Propidium Iodide [NBP2-31155] - Image from verified customer review.
Propidium iodide Solution [NBP2-31155] - Propidium iodide Solution
Cellular Imaging: Propidium Iodide [NBP2-31155] - MDA-MB468 cells stained with Live green dye (Calcein) and dead PI dyes (red) after 5 days of drug treatment. Image from verified customer review.
Formulation, Preparation, and Storage
Preservative
No Preservative
Concentration
Please see the protocols for proper use of this product. If no protocol is available, contact technical services for assistance.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store at 4C in the dark.
Background: Propidium Iodide
Additional Propidium Iodide Products
Product Specific Notices for Propidium Iodide
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.
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