B7-H4 Acts as a T Cell Co-inhibitory Receptor

B7-H4 Binds to an Unidentified Receptor and Inhibits T Cell Activation

Similar to B7-H3, B7-H4 is also highly expressed on various tumor cell types and tumor-associated macrophages and high expression correlates with tumor aggressiveness and reduced survival in a number of different types of cancer.^{1, 2} Although it has yet to be identified, a B7-H4 receptor appears to be expressed on activated T cells and to inhibit T cell proliferation, cytokine production, and cytolytic activity.^3-5 Additionally, B7-H4 signaling is thought to promote the expansion of myeloid-derived suppressor cells (MDSCs), a heterogeneous population of immature myeloid cells with immunosuppressive properties that accumulate in the tumor microenvironment.² Similar to B7-H3, a soluble form of B7-H4 can be generated by ADAM-mediated ectodomain shedding, and high levels of soluble B7-H4 in the blood of cancer patients has been correlated with a poor prognosis.⁶

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B7-H4 binds an unidentified receptor on activated T cells and inhibits T cell proliferation and effector functions.

B7-H4 binds to an unidentified receptor on activated T cells and inhibits T cell functions. Although the receptor for B7-H4 has yet to be identified, research suggests that it binds to a receptor expressed on activated T cells and inhibits T cell proliferation and effector functions.

Analysis of the effect of R&D Systems Recombinant Rat B7-H4 Fc Chimera protein on IFN-gamma secretion by activated T cells.

B7-H4 Inhibits IFN-gamma Secretion by Anti-CD3-Stimulated Human T Cells. Human T cells were treated with an immobilized Mouse Anti-Human CD3 epsilon Monoclonal Antibody (R&D Systems, Catalog # MAB100) and the indicated concentrations of Recombinant Rat B7-H4 Fc Chimera (R&D Systems, Catalog # 10085-B7). IFN-gamma secretion was measured using the Human IFN-gamma Quantikine^TM ELISA Kit (R&D Systems, Catalog # DIF50). The ED₅₀ for this effect is typically 1-6 μg/mL.

Purity of Avi-tag Biotinylated Recombinant Human B7-H4 assessed by capillary electrophoresis (CE-SDS) on the Maurice system.

Assessment of the Purity of Avi-tag Biotinylated Recombinant Human B7-H4 by CE-SDS on the Maurice^TM System. The purity of Avi-tag Biotinylated Recombinant Human B7-H4 Fc Chimera (R&D Systems, Catalog # AVI8870) was assessed by capillary electrophoresis (CE)-SDS on the Maurice System under reducing (R) and non-reducing (NR) conditions and visualized in Compass for iCE software. The gel view is shown as an inset with the relative migration time (RMT) on the electropherogram shown on the far right-hand side of the gel.