Ubiquitin Proteasome System

• Ubiquitin
• Ubiquitin Mutants
• Ubiquitin Chains
• SUMO
• NEDD8
• ISG15
• UFM1
• FAT10

Ubiquitin proteins, mutants, and chains as well as Ubiquitin like proteins SUMO, ISG15, NEDD, UFM, and FAT10. Over 70 ubiquitin proteins available in a wide range of tags. All the essential building blocks to make your in vitro assays a success.

• Single-subunit E3 Ligases
• Multi-subunit E3 Complexes including Neddylated Cullins
• E3 Inhibitors
• PROTACs^® and Targeted Protein Degradation

There are over 600 genes that code for E3 ligases in the human genome. E3 ligases typically confer target specificity for ubiquitination in addition to the enzymatic role of adding ubiquitin. Several E3 ligases have become popular components used with small molecule degraders in the promising field of targeted protein degradation (TPD).

• DUB Enzymes
• DUB Inhibitors

The process of protein ubiquitination is reversible. DUBs are a large class of enzymes that remove ubiquitin from proteins. They play a diverse role across many pathways and often function in direct contact with the proteasome.

• E1 Activating Enzymes
• E1 Inhibitors

E1 enzymes are ubiquitin activating enzymes that catalyze the first step in the ubiquitination process. They bind ubiquitin in an ATP-dependent manner via an internal cysteine residue. This ubiquitin bound to a E1 enzyme is then transferred to an E2 enzyme.

• E2 Conjugating Enzymes
• E2 Inhibitors

E2 enzymes, or ubiquitin-conjugating enzymes are involved in the transfer of ubiquitin to a target protein. There are about 40 human E2 enzymes.

• Proteasome Enzymes
• Proteasome Inhibitors
• Proteasome Substrates

The proteasome is the primary way cells degrade proteins. This mutiprotein complex containing multiple proteases forms a tube-like structure where proteins are degraded as they pass through. Bioactive, purified proteasomes are ideal for studying in vitro degradation of a protein of interest.

• Accessory Proteins

Accessory proteins are kinases and heat shock proteins that act as E3 ligase cofactors and proteins involved in aggresomal disorders such as Tau and alpha-Synuclein. 

• Proteasome Substrates

A wide range of biotinylated and fluorogenic peptide substrates used for in vitro proteasome experiments.

• A Chemical Toolbox to Support PROTACs

• Targeted Protein Degradation Guide

Utilize our ubiquitin-related resources to learn more about the generation of a toolbox of building blocks for the development of Degraders and products available from Bio-Techne to support targeted protein degradation research.