Why RNAscope™ ISH Wins?
RNAscope is the gold-standard for RNA detection due to its ability to uncover added spatial information of biodistribution of the AAV vector and transgene RNA, in single-cells with high sensitivity and specificity. It also enables multiomic detection of protein biomarkers on the same slide, making it an optimal choice.
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Make Smart Choices for Pre-Clinical Programs
Regulatory guidelines from the FDA require assessing biodistribution for cell and gene therapy products by evaluating vector/virus or cellular agent distribution, persistence, and clearance from the administration site to targeted and non-targeted tissues and biological fluids.
RNAscope ISH assay offers the most powerful spatial biology method from the FDA shared options, providing precise tissue-based analysis to aid in preclinical and clinical studies of cell and gene therapy products.
Extracellular Trapping of the AAV Vector
RNAscope Reveals the Complete Picture
Screen AAV vectors with confidence. RNAscope probes enable detection, delivery and uptake of the AAV vector at single-cell sensitivity and accuracy. The image on the right depicts most of the AAV vector is stuck in the interstitial space. This information would not be revealed if a non- spatial method was used.
Fig - RNAscope assay shows vector sequestered in the interstitial space. AAV-treated liver stained with RNAscope LS Red ISH assay (A). HALO overlay (B) identifies AAV-positive nuclei in red and AAV-negative nuclei in white.
Accelerate Approvals of Gene Therapy Pipelines
Confidently Assess the Biodistribution of AAV Vector DNA and Transgene mRNA
RELIABLE
Accurate visual assessment of RNA expression in heterogenous tissues.
SENSITIVE
Deep understanding of the biology to measure functional efficacy and safety testing.
SINGLE-CELL
Measure expression at single-cell and sub-cellular resolution to interrogate if the vector and transgene is in the correct cells.
RNAscope Quantitation Correlates with Other Standard Assays with the Added Spatial Information
Comparison of DKK1 RNAscope with other technologies in multiple different cancer cell lines indicates that the DKK1 RNAscope assay is highly sensitive, specific and accurate with the added SPATIAL information.
Fig - RNAscope assay is highly concordant with other technologies.
The indicated cell types were analyzed for DKK1 RNA expression using RNA-Seq or qPCR (top-panel) and stained for DKK1 RNA by RNAscope (bottom panel). Scale bar: 50 μm.
Citation: Caldwell, C., Rottman, J.B., Paces, W. et al. Validation of a DKK1 RNAscope chromogenic in situ hybridization assay for gastric and gastroesophageal junction adenocarcinoma tumors. Sci Rep 11, 9920 (2021). https://doi.org/10.1038/s41598-021-89060-3
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