Using SDS-PAGE
This simple and sensitive technique uses glycoenzymes to selectively visualize terminal glycans using standard SDS-Page methods.
Using Fluorescence
Bio-Techne provides numerous glycoenzymes to visualize terminal glycans in cultured cells with fluorescence.
Read Our Peer-Reviewed Glycobiology Publications
"Detecting and Imaging O-GlcNAc Sites Using Glycosyltransferases: A Systematic Approach to Study..."
This publication describes a highly specific approach to visualize O-GlcNAc as well as other potential sites for O-GlcNAc modification on biological samples using recombinant glycosyltransferases and chemical probes.
"Imaging specific cellular glycan structures using glycosyltransferases via click chemistry"
This publication describes highly specific approaches for Heparan Sulfate (HS) and T/Tn antigen visualization.
"Probing sialoglycans on fetal bovine fetuin with azido-sugars using glycosyltransferases"
This publication describes a novel Heparanase (HPSE) assay that is very sensitive and highly reproducible.
"Glycoprotein labeling with click chemistry (GLCC) and carbohydrate detection"
This publication describes a method for glycoprotein labeling/carbohydrate detection through glycan replacement termed glycoprotein labeling with click chemistry (GLCC).
"Nonradioactive Glycosyltransferase and Sulfotransferase Assay to Study Glycosaminoglycan..."
This book chapter describes nonradioactive colorimetric assays for glycosyltransferases and sulfotransferases.
"Detecting O-GlcNAc using in vitro sulfation"
This publication describes a method for O-GlcNAc detection using in vitro sulfation using the two GlcNAc sulfotransferases carbohydrate sulfotransferase (CHST) 2 and CHST4 and the radioisotope 35S.
"Golgi-resident PAP-specific 3′-phosphatase-coupled sulfotransferase assays"
This publication describes a highly quantitative and reproducible nonradioactive universal phosphatase coupled sulfotransferase assay.
"Universal phosphatase-coupled glycosyltransferase assay"
This publication describes a highly quantitative and reproducible nonradioactive universal phosphatase coupled glycosyltransferase assay.
"Detection of specific glycosaminoglycans and glycan epitopes by in vitro sulfation using..."
This publication describes a method for detecting glycosaminoglycans (GAGs) in cells and tissue using radiolabeled in vitro sulfation with carbohydrate specific sulfotransferases.
"A versatile polyacrylamide gel electrophoresis based sulfotransferase assay"
This publication describes a novel sulfotransferase assay that uses SDS-PAGE methods.