Targeted Protein Degradation Western Blot Solutions
Simple Western™ provides critical information to run quantitative DC50 curves with a high throughput protein degradation western blot workflow and reproducible and fully quantitative data. Simple Western assays with automated Western blotting capabilities enhance accuracy, ensure data quality, and deliver results. Our systems integrate easily in PROTAC and Molecular Glue Degrader screening assays while providing you the flexibility of seamless transferability of methods and an open platform.
On this page: Literature | Webinars | Additional Resources | Customer Testimonials
The Simple Western Advantage
- Fully automated protein degradation western blot analysis
- Quantitative protein expression data
- Multiplex analysis in chemiluminescence and fluorescence channels
- Stellar™ NIR/IR Modules with industry-leading detection sensitivity
- Process up to 25 samples in 3 hours or 96 samples overnight
- Built-in total protein normalization
Literature
Fully Automated PROTAC Protein Degradation Western Blot Analysis
Fully Automated PROTAC Protein Degradation Western Blot Analysis
In this App Note, researchers performed protein degradation western blot analysis of 120 samples in just two overnight runs to provide highly quantitative data, which allows for an accurate determination of DC50 and Dmax. Researchers used off-the-shelf antibodies and PROTAC® degraders to profile PROTAC®-induced Cereblon neosubstrate degradation.
Validating Targets For Targeted Protein Degradation Using dTAG
Validating Targets For Targeted Protein Degradation Using dTAG
dTAG degradation technology offers a generalizable strategy to degrade, in principle, any intracellular protein of interest. Its broad applicability makes this a valuable strategy for exploring and validating targets using automated western blot protein degradation assays.
Simple Western Protein Degradation Assay for TPD Research
Simple Western Protein Degradation Assay for TPD Research
Do you want immunoassay-like quantitation for your western blot protein degradation analysis with low sample volume? Get picogram-level sensitivity with just 3 µL of starting material! Learn how the fully automated protein degradation assay on Simple Western is used in labs worldwide to quantify Degrader-induced protein knockdown.
Webinars
Accelerating Protein Analysis Throughput at Boehringer Ingelheim
Accelerating Protein Analysis Throughput at Boehringer Ingelheim
The team at Boehringer Ingelheim presents their recent real-world TPD project examples and in-depth analysis, covering insights on device and consumable management and optimized protein degradation assay setups to increase protein degradation western blot throughput.
Automated Western Blot Protein Degradation Research
Automated Western Blot Protein Degradation Research
Watch Dr. Gary Allenby, CSO at Aurelia Bioscience, who shares how their research team utilizes automated protein degradation western blot systems to rapidly and reproducibly quantify protein knockdown following treatment with Degraders.
Targeted Protein Degradation of Lysine Acetyltransferases p300/CBP
Targeted Protein Degradation of Lysine Acetyltransferases p300/CBP
Learn how western blot protein degradation assays with Simple Western reveal dCBP-1 protein degradation of p300/CBP in healthy and diseased cells from Christopher J. Ott, PhD, Harvard Medical School.
Additional Resources
Bio-Techne's Complete Targeted Protein Degradation Workflow Solution
Bio-Techne's Complete Targeted Protein Degradation Workflow Solution
Bio-Techne offers a unique portfolio of high-quality reagents, instruments, and services for your protein degradation pathway research. Our TPD and Induced Proximity Research Product Guide highlights our tools and services to support your Targeted Protein Degradation research.
Benefits of Targeted Protein Degradation With PROTAC® eBook with Wiley
This edition of expert insights highlights advancements in the vibrant field of targeted protein degradation with small molecule drugs that aim for 'undruggable' targets. This eBook also connects researchers to new technologies accelerating drug discovery with robust high throughput screens and quantitative protein assays like the automated Simple Western™ platform from Bio-Techne.
Customer Success Stories
Senior Research Scientist, Aurelia Bioscience
Senior Research Scientist, TCG Lifesciences
