MFI Instruments & Consumables | How Does it Work? | Compare Instruments | Applications
MFI™ gives you more insight into the world of sub-visible particles—way more than just size and count. Because it's image-based, you also get quantifiable morphological parameters for your analysis. Easily differentiate and characterize different particle populations in no time. Plus, MFI has an optic focal length that spans the entire fluidic path, so your sample data is robust and reproducible day in and day out. Add a Bot1 anytime to automate the whole process and kick your throughput up a notch. If you're looking for broader, deeper analysis of your biotherapeutic products, MFI is the particle analysis tool for you.
ProteinSimple is now a brand in the Bio-Techne family of brands that includes R&D Systems, Novus Biologicals and Tocris. As a company, Bio-Techne has developed, manufactured and sold biotechnology products, clinical calibrators and controls, and consumables for protein analysis—and now analytical instrumentation and consumables through ProteinSimple.
MFI Instruments
MFI Virtual Demo
MFI Virtual Demo
See how MFI easily detects, quantitates, and provides morphological data on subvisible particles
How does MFI work?
Micro-Flow Imaging combines the direct imaging capabilities of digital microscopy with the precise control of microfluidics. What does that get you? High-resolution images with 85% sampling efficiency, more precise counts and sizing with full morphological detail for all sub-visible particles in your sample and the complete confidence that you can accurately identify every possible type, from protein aggregates to air bubbles.
Images of the sample are captured as it passes through the flow cell's sensing zone. Every particle in every image is then analyzed to create a database of particle count, size, transparency and morphology (or shape). And you'll have visual verification on the spot, as images are displayed in real-time. You can also display the results for many samples at once, which makes it easy to monitor stability and comparability.
MFI Systems Comparison Chart
|
Description |
MFI 5100 |
MFI 5200 |
|---|---|---|
|
Size Range |
2 to 300 µm |
1 to 70 µm |
|
Percent of Sample Analyzed |
>85% - entire size range |
>85% - entire size range |
|
Depth of Field (DOF) |
400 µm |
100 µm |
|
Flow Cell Depth |
400 µm - DOF matched |
100 µm - DOF matched |
|
Analysis Rate |
200 µL/min |
150 µL/min |
|
Maximum Concentration (@2.5 µm) |
175,000 particles/mL |
900,000 particles/mL |
MFI Applications
Bioprocessing
Enhance the safety and efficacy of your formulation and final product by detecting, quantifying, and classifying different sub-visible particles such as protein aggregates, silicone oil, and other contaminants.
Cell & Gene Therapy
Next-generation analytical instrumentation to help improve and accelerate your cell and gene therapy workflows.
Protein Aggregation & Particle Analysis
Improve the safety of your therapeutic with accurate detection and characterization of translucent protein aggregates and other particulates.
Biosimilars
Be assured of your biosimilar’s stability through the reproducible detection and quantitation of particulate matter and aggregates during development, formulation, and manufacturing.
Vaccine Development
Develop and manufacture safe vaccines by accurately visualizing and classifying different particle contaminants in your product, from development to delivery.