Lentiviral Vector Characterization Made Easy with Maurice CE-SDS

The accurate characterization of lentiviral vectors (LVVs) for various attributes such as purity, identity, viral titer, and safety is critical for successful gene therapy development. Even today, LVVs are characterized for some of their attributes with techniques such as SDS-PAGE and ELISA. SDS-PAGE is a manual and laborious method that often comes with reproducibility issues, while ELISAs can overestimate the viral titer present.

To address the challenges presented by these analytical techniques, this application note demonstrates a robust and automated CE-SDS method on the Maurice™ instrument, which can be used as both an identity assay as well as a titer assay for LVV analysis.