Application Brief 1001: p-EGFR NanoPro Assay

The epidermal growth factor receptor (EGFR) is a transmembrane receptor for the epidermal growth factor family (EGF-family) of extracellular protein ligands. Ligand binding activates its intracellular tyrosine kinase domain resulting in auto as well as substrate phosphorylation. Mutation and deregulation of EGFR is implicated in many cancer types. The data shows a time dependent change in anti-phospho EGFR (Y1068) antibody signal in HeLa cells in response to EGF treatment.