Application Brief 1009: Hsp70 Loading Control NanoPro Assay

The 70 kilodalton heat shock proteins (Hsp70) are a family of ubiquitously expressed heat shock proteins. Proteins with similar structure exist in virtually all living organisms. The Hsp70s are an important part of the cell's machinery, and help to protect proteins from misfolding under stress. We describe its use as loading control for EGF stimulation in HeLa and MCF10A cells. In the NanoPro assay, Hsp-70 is present as a single peak around pI 5.8 under the conditions described.