Application Brief 1019: p-JNK NanoPro Assay

c-Jun N-terminal kinases (JNK), originally identified as kinases that bind and phosphorylate c-Jun on Ser63 and Ser73, are mitogen-activated protein kinases which are responsive to stress stimuli, such as cytokines, UV-irradiation, heat shock, and osmotic shock, and are involved in T cell differentiation and apoptosis. JNK1, 2 and 3 share a total of 10 isoforms with pI's ranging from 5.4-7.6. All three JNK kinases share a similar Thr/Tyr phosphorylation site (T183/Y185). We evaluate change in JNK phosphorylation in UV-treated HEK293 cells and imatinib-treated K562 cells using a dual phospho-antibody against that site.