Application Brief 1024: c-Myc Epitope Tag NanoPro Assay

Epitope tags are widely used for afinity purification as well as for highly sensitive detection of recombinant proteins. The c-Myc-tag consists of a short peptide sequence (MEEQKLISEEDLLM). EGFP was expressed with a c-Myc-tag at either the C- or N-terminus of the protein. As expected from the amino acid composition of this tag, a slight acidic shift in the pI of the tagged protein can be observed.