What if you could confirm the identity of your AAVs, assess their stability, and estimate empty/full capsid content all on the same instrument? This poster describes an icIEF platform method for the charge heterogeneity analysis of denatured AAV capsid proteins using native fluorescence and UV absorbance detection modes. By analyzing two commercially available percent genome empty/full capsid series and leveraging the two detection modes on the Maurice™ system, the quantification from the empty/full series can be used to estimate the fullness of unknown samples.
