Charge Variant Characterization of Innovator & Biosimilar Drugs with MauriceFlex & BioAccord LC-MS System

This application note presents an efficient workflow for the comparative analysis of charge variants in a monoclonal antibody and a research-grade biosimilar. The workflow involves: 

• Charge separation and icIEF-based fractionation on the MauriceFlex™ System 
• LC-MS analysis using the BioAccord™ System (Waters Corporation), with no additional sample prep required after fractionation. 
• Uncovering key differences between the two samples, including light chain fragmentation and C-terminal lysine residues.