Combining the RNAscope ISH Technology with IHC to spatially resolve RNA and protein targets simultaneously

The combined RNAscope ISH IHC/IF workflow can detect RNA and protein targets on the same sample with high sensitivity and specificity at the single cell level while preserving the tissue morphology. This report presents the applications of the dual ISH IHC/IF workflow in neuroscience, immuno-oncology, and beyond. This technique can be used to:

• Characterize immune cells
• Identify the cellular source of secreted proteins
• Visualize neuronal cell type markers
• Confirm trafficking and activation of CAR T cells