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Precision Cytokine Detection & Analysis with an Automated ELISA Platform

Application Notes

As small soluble protein messengers released by cells, cytokines interact and communicate with other nearby cells. The results are pleiotropic and affect cellular function in ways that can indicate inflammation or disease progression, making cytokine detection, measurement, and monitoring important to clinicians and researchers alike. The enzyme-linked immunosorbent assay (ELISA) is the traditional way to detect and validate protein biomarkers in serum, cell preparations, or other biological sample types because the assay offers high target specificity.

However, the standard ELISA approach doesn’t always have the sensitivity or reproducibility required to detect endogenous levels of an analyte reliably. Moreover, analyte cross-reactivity complicates multiplexing, and the tedious manual workflow that calls for hours of time to produce a result followed by hours of time for analysis has necessitated change and produced an improved, alternative approach that streamlines the entire process. Learn how scientists are using Simple Plex™ assays on Ella™ to do everything an ELISA does but faster and with greater sensitivity and reproducibility.

 

cytokine detection analysis application note mockup