Expression of Mature microRNAS, miR-1287-5p and miR-658, is Upregulated in Diabetic Kidney Disease (DKD) Tissues
Application NotesmiRNAs are endogenous, small non-coding RNAs that can regulate the expression of mRNAs post-transcriptionally either via translational repression or mRNA degradation. Dysregulation of miRNA expression is observed in various diseases and altered physiological states, for example in Diabetic Kidney Disease (DKD) where mechanisms underlying progression of DKD to End Stage Kidney Disease (ESKD) are not fully understood.
In this application note we highlight a study by Eiichiro Satake et al., in which the authors identified a signature of 17 miRNAs and 6 axon guidance pathway proteins that were robustly associated with an increased 10-year risk of ESKD in Type 1 and Type 2 diabetes. Using miRNAscope™ in situ hybridization assay, localization and levels of two exemplar miRNAs, miR-1287-5p and miR-658 were detected in tissue affected by DKD, but not in normal kidney tissue.
The miRNAscope Assay is an RNA in situ hybridization assay enabling highly robust detection of miRNAs, siRNAs, ASOs, and other smaller RNAs (17-50nt) in tissues with spatial and morphological context at single cell resolution. Compared other available tools for small-RNA analysis, the miRNAscope assay drastically improves the detection sensitivity and specificity of small-RNA species in various sample types across different tissues, thus filling a critical gap in spatial analysis of miRNA expression.
