A Fully Automated Capillary Electrophoresis System for Western Analysis Using On-Line Stacking as an Easy Tool for the Enhancement of Sensitivity and Resolution
Life Science Posters
Size-based characterization of proteins has been predominately performed by either SDS-PAGE/Western blot analysis or by capillary electrophoresis (CE). Each technique has advantages – Western blotting exploits high sensitivity as well as specificity of antibody binding, and CE offers high resolution and reproducibility. The sensitivity and resolution of the results obtained from either method is often challenged by the ability to pre- concentrate or stack enough protein sample before separation. Simon, a new size-based separation platform that runs Simple Western assays, combines for the first time the advantages of both Western blotting and CE into a single automated workflow. The work presented illustrates the dependency of stacking efficiency upon the plug length of the sample and stacking matrix. Optimization of stacking conditions resulted in a significant sensitivity and resolution increase using the Simple Western assay.
