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High-throughput automated protease-free workflows for RNA-protein co-detection using RNAscope technology

by Qi Shen, Renzo Adilardi, Emerald Doolittle et al.

Life Science Posters

Spatial modalities that allow RNA and protein detection on the same tissue, enable simultaneous detection of RNA biomarkers and identification of cell types expressing those target biomarkers. RNAscope is an established leader in RNA biomarker detection with high-performing high-throughput automated ISH assays. To enable simultaneous detection of protein, we have developed a protease-free workflow for RNA-Protein co-detection by in situ hybridization followed by immunohistochemistry (ISH-IHC). This poster demonstrates the protease-free workflow using two different systems: the BOND Rx using the Chromogenic red assay by combining PCA3 RNA with CD3 and CD8 proteins in prostate cancer tissue, and Roche’s Ventana Discovery Ultra platform, using the IgK RNA and CD20 protein in tonsil tissue and VEGF RNA and CD31 protein in lung cancer. The findings indicate that the protease-free workflow significantly improved the performance of protease-sensitive antibodies such as CD8, CD31 and CD20 while maintaining the RNA signal quality and preserving tissue morphology. 

Image of High-throughput automated protease-free workflows for RNA-protein co-detection using RNAscope technology poster