A Novel icIEF Fractionation & SPR-Based Workflow for Correlating the Charge Structure to the Function of a Bispecific Antibody
Application NotesThis app note demonstrates a novel, streamlined workflow for characterizing biotherapeutic charge variants. Discover the powerful combination of icIEF fractionation on the MauriceFlex™ System and SPR on the Alto™ System (Nicoya Lifesciences).
- Learn about simplified workflows: See how charge variant fractions are collected on the MauriceFlex System and loaded onto the Alto System for binding analysis.
- Explore charge-based binding analysis: See how individual charge variants of Mosunetuzumab and a biosimilar interact with CD3 and CD20 ligands.
- Correlate structural and functional data: Examine how charge variant structures relate to binding properties, with insights confirmed by LC-MS analysis.
