Immunohistochemistry (IHC) Handbook

Immunohistochemistry (IHC) is a widely used immunostaining technique using antibodies to detect an antigen of interest in the cells of a fixed tissue section and provides semi-quantitative data about target protein expression, distribution, and localization.

Our recently updated handbook offers a comprehensive introduction to IHC that is well-suited for researchers new to the technique and experienced scientists looking for a quick refresher.

Download our NEW handbook and learn about:

• Different variables to consider when performing an IHC experiment
• Multiplexing IHC
• RNA-Protein co-detection and the benefits of in situ hybridization (ISH)-IHC
• Standard IHC workflow including sample preparation and fixation, antigen retrieval, blocking non-specific binding, antibody selection, and detection methods
• Controls for IHC
• Easy-to-follow IHC staining protocols (chromogenic and fluorescent)
• Troubleshooting tips
• Buffer and reagent recipes