Insights on Dual ISH-IHC - Guidance from the experts on performing RNAscope™ ISH and immunohistochemistry on the same sample

Analysis of both RNA and protein provides invaluable information for understanding the regulation of expression. In situ hybridization (ISH) and immunohistochemistry (IHC) share common features that make them unique amongst protein and RNA detection technologies.

•     Morphological context: detection of RNA and protein while conserving the cell and tissue structural integrity 
•     Similar workflows from sample fixation, pre-treatment, hybridization, signal amplification and data analysis.

Due to the multiple benefits of running both ISH and IHC and the robustness of the RNAscope™ assay, some customers developed a dual ISH-IHC protocol to combine both assays on the same sample slide. We conducted interviews with four researchers who share their insights and protocols about using the dual ISH-IHC technique.

Download our Application Note and learn from these experts how to develop a dual ISH-IHC protocol.

Interview with:

• Kristie Wetzel, Novartis Institute for

  BioMedical Research, Cambridge, MA, USA.

• Dr Bradley Spencer-Dene, The Francis Crick

  Institute, London, UK.

• Wanda Wang, University of Pittsburgh, PA,

  USA.

• Mike Millar, Queen’s Medical Research

  Institute, University of Edinburgh,

  Edinburgh, UK.