Multi-Omic Spatial Analysis with Simultaneous Detection of Small RNAs, mRNAs and Proteins

Oligonucleotide therapies leverage the regulatory mechanisms of miRNAs and other small RNAs, such as antisense oligos (ASOs) and silencing RNAs (siRNA). Ensuring the safety and efficacy of these therapies requires sensitive spatial techniques to assess the biodistribution of vectors and introduced small RNAs, and to identify specific cell types. 

In this study, we used spatial multi-omic methods to simultaneously visualize miR-205 expression and associated tumor target genes (PanCK, PTEN, and TP53), alongside cell-type specific markers, using antibody-based protein detection. This was performed on head and neck cancer tumor samples using the novel RNAscope™ Plus smRNA-RNA Assay, which detects one small RNA and up to three mRNA targets with TSA-based fluorescent readouts. 

This analysis offers deep insights into miRNA-driven disease pathology while also enabling precise assessment of the biodistribution and efficacy of oligonucleotide therapeutics, with single-cell resolution.

Download this multi-omic spatial analysis poster to uncover: 

• Simultaneous detection of miRNA and RNA targets across diverse samples 

• Multi-omic codetection of small RNAs, mRNAs, and proteins in tumor tissues  

• How the RNAscope Plus assay provides insights into tumor initiation, progression, and angiogenesis