Multi-omic spatial interrogation of tumor-infiltrated immune cells using the RNAscope™ Co-detection assay and the new TSA Vivid™ dyes

The new RNA-protein Co-detection workflow improves IHC/IF antibody compatibility with the RNAscope ISH technology creating an extremely powerful multiomic solution to elucidate cellular heterogeneity, identify novel cell populations while retaining spatial information with morphological context.

• Using the RNAscope RNA protein co-detection assays we assessed the tumor microenvironment and characterized tumor infiltrated immune cells.
• We identified immune cell sub-populations using cell marker-specific antibodies and detected expression of activating chemokines and cytokines using RNAscope ISH.
• With the new Multiplex Fluorescent v2 assay with TSA Vivid™ dyes, low-abundance cytokines were visualized with increased signal intensity and resolution.  
• The co-detection reagents and workflow are compatible with manual and automated platforms and can be combined with most RNAscope, BaseScope and miRNAscope assays