Optimize Your icIEF Fractionation Method on the MauriceFlex System
Application NotesRead this tech note to learn about optimizing fraction collection on the MauriceFlex™ system:
- The ability of a plate reader to read the fractions in wells containing low concentrations of charge variants after fractionation can be challenging due to its poor sensitivity.
- The Simple Western pI markers can illuminate bright fluorescence signals on a plate reader. Spiking SW pI markers with pI values that sandwich the charge variants of interest helps in reducing the number of fractions reanalyzed on Maurice™.
- This optional approach can reduce guesswork in fraction identification and save time in fraction reanalysis.