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Optimize Your icIEF Fractionation Method on the MauriceFlex System

Application Notes

Read this tech note to learn about optimizing fraction collection on the MauriceFlex™ system

  • The ability of a plate reader to read the fractions in wells containing low concentrations of charge variants after fractionation can be challenging due to its poor sensitivity​. 
  • The Simple Western pI markers can illuminate bright fluorescence signals on a plate reader. Spiking SW pI markers with pI values that sandwich the charge variants of interest helps in reducing the number of fractions reanalyzed on Maurice™.​ 
  • This optional approach can reduce guesswork in fraction identification and save time in fraction reanalysis. 

Optimize icIEF Fractionation Application Note