Quantitative Multi-Analyte Detection of Key Cytokine Release Syndrome Biomarkers

We designed a quantitative multi-analyte detection assay of key cytokine release syndrome (CRS) biomarkers. This µfluidic parallel immunoassay measured four cytokines simultaneously in only one hour. We compared cytokine measurements for human IL-6, IL-1β, TNF-α, and IFN-γ between our Quantikine ELISA and Simple Plex™ immunoassays using an in vitro model for CRS.