RNAscope® Manual Solutions Brochure: Biomarker Analysis by RNA in situ hybridization: Get quantitative molecular detection with morphological context in a single assay

RNAscope® is a proprietary RNA in situ hybridization (ISH) assay based on ACD patented signal amplification and background suppression technology which advances RNA biomarker analysis in tissues and cells. Unique to this technology, RNAscope® delivers quantitative, sensitive and specific molecular detection of RNA species on a cell-by-cell basis with morphological context in a single assay. This enables researchers to visualize which genes are expressed, localize where they are expressed, and quantify the level of expression.

Key Benefits:

Unprecedented Sensitivity

Detection of a single RNA molecule requires only three double Z probe pairs to bind to the target RNA molecule. The RNAscope® 20 double Z probe pairs design provides robustness against partial target RNA accessibility or degradation.

Specific Molecular Detection

Signal amplification coupled with simultaneous background suppression strategy results in single-RNA-molecule detection even in partially degraded samples. Rigorous double Z probes design eliminates cross hybridization to unintended targets and routinely distinguishes RNA sequences with up to 85% homology.

Morphological Context

Provides cell-specific expression information in intact tissue architecture.

Quantitative

Single-molecule detection at single-cell resolution enables researchers to quantify per cell expression level across heterogeneous cell populations.

Universal

Works for virtually ANY gene from ANY species in ANY tissue