RNAscope Reference Guide

This comprehensive reference guide presents guidelines, protocols, and data examples for obtaining optimal RNA in situ hybridization results with any tissue type from any species.

• The RNAscope™ workflow
• The importance of controls
• Choosing appropriate control probes
• Materials and methods
• Optimal pretreatment conditions for different tissues in different species

In addition, this eBook contains detailed discussions and recommendations related to:

Optimizing results by species and tissues

• Comparison of mild and standard pretreatment conditions
• Positive and negative control probe data
• Proliferation and apoptosis marker analysis

Troubleshooting

• Sample preparation and optimization
• Unexpected staining patterns
• High background
• Tissue detachment
• Other issues