RNAscope™ Tissue Profiling of Immuno-Oncology Checkpoint Controls in the Tumor Microenvironment

Recent clinical successes with immune checkpoint blockade have provided promising immune-based therapeutic approaches for controlling malignancy. However, it is becoming increasingly apparent that the cellular composition and interactions between cells within the tumor microenvironment (TME) are extremely complex and varied. The RNAscope™ technology is an advanced RNA in situ hybridization (ISH) assay that enables combinatorial visualization and quantification of cell-specific gene expression, targeting RNA sequences directly in tissues.

We examined the co-expression of multiple immune, checkpoint, and functional markers in 63 NSCLC tumor and non-tumor tissues using the RNAscope technology and compiled these results into a comprehensive dataset consisting of 40x Aperio image files and digital image analysis for quantification of cellular expression.