Spatial multiplex profiling of immune cell markers in FFPE tumor tissues using the RNAscope™ HiPlex v2 in situ hybridization assay
Life Science Posters
The tumor microenvironment (TME) is highly complex, comprised of tumor cells, immune cells, stromal cells, and extracellular matrix. Understanding spatial interactions between various cell types and their activation states in the TME is crucial for implementing successful immunotherapy strategies against various types of cancer. Here we demonstrate a newly developed HiPlex v2 assay with capability of iteratively multiplexing up to 12-48 targets in FFPE, fixed and fresh frozen samples.
In conclusion, this study demonstrates a highly sensitive and specific multiplexed technique, the RNAscope HiPlex v2 ISH assay for gene expression profiling of target genes to assess immune regulation in FFPE tumor tissues. By expanding HiPlex capabilities to FFPE tissues, RNAscope HiPlex v2 image analysis and quantification provides a highly resolved spatial multiplexing solution to interrogate complex tissues and investigate biologically meaningful questions.
