Using Single-Cell Westerns to Validate Single-Cell RNSeq Data

Single-cell gene expression studies are revolutionizing our understanding of heterogeneity in disease. Single-cell RNA sequencing tools are powerful for discovering mRNA transcript heterogeneity. However, mRNA levels do not always correlate with functional protein levels. In this application note, learn how Milo was used in parallel with a single-cell RNSeq workflow at the Stanford Functional Genomics Facility to validate single-cell RNA expression studies with single-cell protein expression data and ensure accurate and complete conclusions about cellular function. Because it uses the large Western catalog of antibodies & can easily measure intracellular proteins, Milo is the only platform with the versatility to detect diverse targets that are discovered in a sequencing run.