Webinar: Western Blotting Optimization

Understanding the Variables to Optimize Your Western Blots - Paul Joseph, MS

In this webinar, Paul Joseph M.S. covers the Western Blot workflow including sample preparation, protein size fractionation via gel electrophoresis, protein transfer to membrane, and immunodetection. The presentation also details the steps that can be optimized during the workflow. One of the key takeaways is that great detection requires great transfer. This webinar goes in depth into optimizing protein transfer based on protein size and chemical nature. Following protein transfer, additional variables for improving immunodetection are covered such as blocking solution choice, primary and secondary antibody selection, type of wash solution, and the detection method. Other topics covered include the importance of using a loading control, antibody titration, and the importance of using high quality, reproducible antibodies. Lastly, the webinar closes on Protein Simple instruments and the utility of using automated Simple Western^TM instruments.