Lightning-Link (R) HRP: Conjugation Kits

Easy HRP labeling of your antibody with 30 seconds hands-on time. Antibodies are labeled to enzymes in order to amplify the signal via the catalytic properties of the enzyme. Horseradish peroxidase (HRP) labeling is one of the most commonly used enzymes for this purpose. HRP conjugates are ideal for use in ELISA, immunoblotting and immunohistochemistry applications. HRP has a faster catalytic rate than alkaline phosphatase (AP) and is more sensitive.

The Lightning-Link conjugation system represents a quantum leap forward in conjugation technology. It allows you to make antibody conjugates with minimal hands-on time - less than 30 seconds. Lightning-Link simplifies immunoassay techniques, such as western blotting, ELISA and immunohistochemistry, by eliminating secondary reagents and cutting the number of incubation and wash steps.

Despite its simplicity, Lightning-Link is a very sophisticated conjugation system in which the antibody is directionally coupled to the label (and not to itself) in a controlled fashion, creating high quality conjugates.

As the procedure is not interrupted by desalting steps, trial conjugates can be prepared with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.with microgram quantities of protein and then scaled up with ease. Lightning-Link eliminates problems associated with scale up; hands-on time is essentially independent of process scale. Consistency from batch to batch is easy to achieve, and recoveries approach 100%.