Proteome Profiler Mouse Apoptosis Array

A membrane-based antibody array for the parallel determination of the relative levels of mouse apoptosis-related proteins. Validated for analyte detection in cell lysates.

Principle of the Assay

The Proteome Profiler Mouse Apoptosis Array Kit is a membrane-based sandwich immunoassay. Capture antibodies spotted in duplicate on nitrocellulose membranes bind to specific target proteins present in the sample (Step 1). Captured proteins are detected with biotinylated detection antibodies (Step 2) and then visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of the bound analyte.

Why Use an Antibody Array to Detect Apoptosis-Related Proteins?

Determining the expression of multiple proteins in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple proteins in a single sample can be cost-effective and also save time and sample.

• Rectangular 8-Well Multi-dish
• 8 Mouse Apoptosis Array nitrocellulose membranes spotted with 21 different antibodies to mouse apoptosis-related proteins
• Array Buffer 1
• Array Buffer 8
• Lysis Buffer 17
• Chemi Reagent 1
• Chemi Reagent 2
• Detection Antibody Cocktail, Mouse Apoptosis Array
• Streptavidin-HRP
• Transparency Overlay Template
• Wash Buffer Concentrate (25X)

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.

Western blotting antibodies for analytes related to the Mouse Apoptosis Array Kit

Scientific Data Examples for Proteome Profiler Mouse Apoptosis Array

	Expression of Mouse Apoptosis-related Proteins in Cell Lysate. Figure 1A: A20 mouse rectal carcinoma cell lysate (650 μg). Figure 1B: SW/3T3 Swiss albino mouse embryo fi broblast cell lysate (300 μg). Figure 1C: Ba/F3 mouse lymphocyte cell lysate (400 μg). Figure 1D: Hepa 1-6 mouse liver epithelial cell lysate (650 μg).
	Expression of Mouse Apoptosis-related Proteins in Cell Lysate. Figure 2: Lysates from C2C12 mouse muscle myoblast cells were left untreated or treated with 1 μM STS for 3 hours.

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