5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24452

Novus Biologicals, part of Bio-Techne
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NBP3-24452
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and animal tissue

Sensitivity

28.0 U/L

Assay Range

28.0 - 581 U/L

View all 5'-Nucleotidase/CD73 Activity Assays »

Product Specifications

Kit Type

Activity Assay Kit (Colorimetric)

Reactivity

Non-species specific

Description

Detection instrument: Microplate reader (550 nm)
Assay time: 30 min

Precision

Intra-Assay Precision (Precision within an assay) %CV < 3.3

Inter-Assay Precision (Precision between assays) %CV < 6

Recovery for 5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric)

Recovery

Average recovery rate: 105%

Scientific Data Images for 5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric)

5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric)

5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric) [NBP3-24452] -

5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric) [NBP3-24452] - Standard Reference Curve
5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric)

5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric) [NBP3-24452] -

5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric) [NBP3-24452] - Assay Example

Kit Contents for 5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric)

  • Microplate
  • Plate Sealer
  • Reagent 1: Working Solution
  • Reagent 2: Chromogenic Agent
  • Reagent 3: 0.6 mmol/L Inosine Standard Solution

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: 5'-Nucleotidase/CD73

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.[supplied by OMIM]

Alternate Names

5-NT, CD73, E5NT, eNT, NT5E, NTE

Gene Symbol

NT5E

Additional 5'-Nucleotidase/CD73 Products

Product Documents for 5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for 5'-Nucleotidase/CD73 Activity Assay Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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