FlowTACS Flow Cytometry Apoptosis Detection Kit

Name: FlowTACS Flow Cytometry Apoptosis Detection Kit
Brand: R&D Systems
SKU: 4817-60-K

R&D Systems, part of Bio-Techne | Catalog # 4817-60-K

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Citations (15)

Product Datasheet / COA

Identification and quantitation of fixed apoptotic cells in suspension by Flow Cytometry.

Key Product Details

Features:	Provides an in situ labeling technology to identify and quantitate cultured apoptotic cells by TUNEL-based and flow cytometry.
Key Benefits:	Identification and quantitation of fixed apoptotic cells in suspension by flow cytometry. Allows bi-color and tri-color analysis. Designed for use with fixed cells. Includes exclusive non-enzymatic Cytonin permeabilization reagent. Includes TACS-Nuclease solution for preparing sample-dependent positive controls.

Technical Data
Citations / Reviews

Summary for FlowTACS Flow Cytometry Apoptosis Detection Kit

Why Use the FlowTACS Flow Cytometry Apoptosis Detection Kit?

FlowTACS also provides flexibility in selection of fluorophores that are compatible with your research design. The kit allows multi-color labeling in conjunction with experiment-specific antibodies.

Kit Contents for FlowTACS Flow Cytometry Apoptosis Detection Kit

Streptavidin-FITC
TACS-Nuclease
TACS-Nuclease Buffer
/TACS 2 TdT dNTP
TdT Enzyme
50x Manganese Cation
10X TdT Labeling Buffer
10X TdT Stop Buffer
PI/RNase
Cytonin

Product Specifications for FlowTACS Flow Cytometry Apoptosis Detection Kit

Species

Multi-species

Scientific Data Examples for FlowTACS Flow Cytometry Apoptosis Detection Kit

	Analysis of DNA Fragmentation in Cycloheximide- or Dexamethasone-treated Murine Thymocytes. Murine thymocytes were treated with 10 µg/mL cycloheximide (A) or 1 µM dexamethasone (B) for 16 hours after which the cells were harvested, labeled according to the FlowTACS protocol (Catalog # 4817-60-K), and analyzed by flow cytometry. The R1 populations are apoptotic. Data were provided courtesy of N. Hardegen, NIH, NIDR, Bethesda, MD.

Preparation & Storage

Shipping Conditions	The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

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Product Documents for FlowTACS Flow Cytometry Apoptosis Detection Kit

Product Datasheets

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