TumorTACS In Situ Apoptosis Detection Kit

Name: TumorTACS In Situ Apoptosis Detection Kit
Brand: R&D Systems
SKU: 4815-30-K

R&D Systems, part of Bio-Techne | Catalog # 4815-30-K

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Citations (23)

Product Datasheet / COA

In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.

Key Product Details

Features:	A complete TUNEL assay based kit tailored for the detection of DNA fragmentation associated with apoptosis in tumors or cancer cells.
Key Benefits:	Unique buffer system produces more consistent labeling. Performance tested on tumor samples. Includes both Proteinase K and exclusive non-enzymatic Cytonin permeabilization reagent. Includes TACS-Nuclease solution for preparing sample-dependent positive controls. Helps resolve unique problems encountered when using tissues or cells from tumors.

Technical Data
Citations / Reviews

Summary for TumorTACS In Situ Apoptosis Detection Kit

Why Use TumorTACS In Situ Apoptosis Detection Kit?

The labeling of tumor-containing specimens can be problematic due to the high levels of necrosis in these tissues. During necrosis, cessation of homeostasis, cell swelling, and nuclear membrane rupture generates highly degraded DNA that can interfere with interpretation of data.

Product Specifications

In situ detection of apoptosis in fixed frozen, paraffin embedded, or plastic embedded cells and tissues.
Assists in the identification of apoptotic morphologies.

Kit Contents for TumorTACS In Situ Apoptosis Detection Kit

Proteinase K
Streptavidin-HRP
Diaminobenzidine
DAB Enhancer
TACS-Nuclease
TACS-Nuclease Buffer
Methyl Green 1%
TACS 2 TdT Labeling Buffer
TACS 2 TdT Stop Buffer
TACS 2 TdT dNTP
TdT Enzyme
50x Manganese Cation
Cytonin

Scientific Data Examples for TumorTACS In Situ Apoptosis Detection Kit

	*Apoptotic Cells within a Mouse Mammary Tumor Identified using the TumorTACS In Situ* Apoptosis Detection Kit.** Mammary tumor tissue was fixed in 4% paraformaldehyde overnight and paraffin-embedded. Five micron sections were prepared and placed onto glass microscope slides. The sample was processed following the TumorTACS Kit protocol (Catalog # 4815-30-K). Brown stained nuclei indicate apoptotic cells.

Preparation & Storage

Shipping Conditions	The components for this kit may require different storage/shipping temperatures and may arrive in separate packaging. Upon receipt, store products immediately at the temperature recommended on the product labels.
Storage	Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

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Product Documents for TumorTACS In Situ Apoptosis Detection Kit

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