Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Bovine, Canine, Equine

Cited:

Human, Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Knockout Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3G12

Concentration

1 mg/ml

View all 14-3-3 eta Antibodies »

Product Specifications

Immunogen

Full length recombinant human 14-3-3 eta expressed in and purified from E.coli [UniProt# Q04917].

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for 14-3-3 eta Antibody (3G12)

Western Blot: 14-3-3 eta Antibody (3G12) [NBP1-92691]

Western Blot: 14-3-3 eta Antibody (3G12) [NBP1-92691]

Western Blot: 14-3-3 eta Antibody (3G12) [NBP1-92691] - HEK293T, MDA-MB-231 and MCF-7 whole cell lysates, 30 ug/lane. 10% SDS-PAGE. Antibody at 1:1000, 4C overnight. WB image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: 14-3-3 eta Antibody (3G12) [NBP1-92691]

Immunocytochemistry/ Immunofluorescence: 14-3-3 eta Antibody (3G12) [NBP1-92691]

Immunocytochemistry/Immunofluorescence: 14-3-3 eta Antibody (3G12) [NBP1-92691] - Analysis of HeLa cells stained with mouse mAb to 14.3.3, NBP1-92691, dilution 1:1,000 in red. Blue is DAPI staining of nuclear DNA. The NBP1-92691 antibody reveals the diffuse cytoplasmic distribution of 14.3.3 protein with higher concentration in the perinuclear region.
Western Blot: 14-3-3 eta Antibody (3G12) [NBP1-92691]

Western Blot: 14-3-3 eta Antibody (3G12) [NBP1-92691]

Western Blot: 14-3-3 eta Antibody (3G12) [NBP1-92691] - analysis of neural tissue and cell lysates using Mouse mAb against 14-3-3n ( green). [1] protein standard, [2] Rat whole brain, [3] Mouse whole brain, [4] NIH/3T3, [5] Hek293, [6] HeLa, [7] SH-SY5Y, [8] C6 cells.

Applications for 14-3-3 eta Antibody (3G12)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Immunohistochemistry

1:1000

Western Blot

1:5000
Application Notes
This 14-3-3 eta (3G12) antibody is useful for Immunocytochemistry/Immunofluorescence, immunohistochemistry, and Western blot, where a band can be seen at approximately 28 kDa. Knockout validation (PMID: 31640562).

Reviewed Applications

Read 1 review rated 5 using NBP1-92691 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

50% PBS, 50% glycerol

Preservative

0.035% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 14-3-3 eta

14-3-3 proteins are highly conserved and ubiquitously expressed proteins present as multigene families in most of the living organisms wherein they play a key role of bringing signal transduction to completion, and implicates in regulating several cellular processes including apoptosis, cell-cycle checkpoints and nutrient-sensing signaling pathways. 14-3-3 proteins have several isoforms which include -beta, -gamma, -epsilon, -sigma, -zeta, -tau, and -eta. 14-3-3 proteins can form homo- or heterodimers which interacts with a wide variety of proteins such as transcription factors, enzymes/kinases, cytoskeletal proteins, phosphatases, and other signaling molecules. 14-3-3 interactions with its targets occurs primarily via phospho-Ser/Thr motif, however, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation-independent interactions have been shown. 14-3-3 binding masks specific sequences of target proteins, thereby altering their localization, phosphorylation state, stability, and molecular interactions, and also induces target protein conformational changes which results in altered target protein function. Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in developmental and disease signaling which underlies their different functions despite sequence similarities. 14-3-3 eta has been shown to accumulate in CSF of Creutzfeld Jacob Disease patients; bind to alpha-synuclein in Lewy bodies of Parkinson's disease, and has been linked to early-onset Schizophrenia.

Alternate Names

1433 eta, Protein AS1, YWHA1, YWHAH

Entrez Gene IDs

7533 (Human)

Gene Symbol

YWHAH

UniProt

Q04917

Additional 14-3-3 eta Products

Product Documents for 14-3-3 eta Antibody (3G12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for 14-3-3 eta Antibody (3G12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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