Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Canine, Equine

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Knockout Validated, Western Blot

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 3G12

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all 14-3-3 eta Antibodies »

Product Specifications

Immunogen

Full length recombinant human 14-3-3 eta expressed in and purified from E.coli [UniProt# Q04917].

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Applications for 14-3-3 eta Antibody (3G12) [FITC]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Knockout Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This 14-3-3 eta (3G12) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at approximately 28 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: 14-3-3 eta

14-3-3 proteins are highly conserved and ubiquitously expressed proteins present as multigene families in most of the living organisms wherein they play a key role of bringing signal transduction to completion, and implicates in regulating several cellular processes including apoptosis, cell-cycle checkpoints and nutrient-sensing signaling pathways. 14-3-3 proteins have several isoforms which include -beta, -gamma, -epsilon, -sigma, -zeta, -tau, and -eta. 14-3-3 proteins can form homo- or heterodimers which interacts with a wide variety of proteins such as transcription factors, enzymes/kinases, cytoskeletal proteins, phosphatases, and other signaling molecules. 14-3-3 interactions with its targets occurs primarily via phospho-Ser/Thr motif, however, binding to divergent phospho-Ser/Thr motifs, as well as phosphorylation-independent interactions have been shown. 14-3-3 binding masks specific sequences of target proteins, thereby altering their localization, phosphorylation state, stability, and molecular interactions, and also induces target protein conformational changes which results in altered target protein function. Distinct temporal and spatial expression patterns of 14-3-3 isoforms have been observed in developmental and disease signaling which underlies their different functions despite sequence similarities. 14-3-3 eta has been shown to accumulate in CSF of Creutzfeld Jacob Disease patients; bind to alpha-synuclein in Lewy bodies of Parkinson's disease, and has been linked to early-onset Schizophrenia.

Alternate Names

1433 eta, Protein AS1, YWHA1, YWHAH

Gene Symbol

YWHAH

Additional 14-3-3 eta Products

Product Documents for 14-3-3 eta Antibody (3G12) [FITC]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for 14-3-3 eta Antibody (3G12) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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