Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Canine, Equine

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Knockout Validated, Western Blot

Label

DyLight 755 (Excitation = 754 nm, Emission = 776 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 3G12

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all 14-3-3 eta Antibodies »

Product Specifications

Immunogen

Full length recombinant human 14-3-3 eta expressed in and purified from E.coli [UniProt# Q04917].

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Applications for 14-3-3 eta Antibody (3G12) [DyLight 755]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Knockout Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This 14-3-3 eta (3G12) antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at approximately 28 kDa.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: 14-3-3 eta

Members of the 14-3-3 family of proteins are highly conserved proteins, localized in neurons, and are axonally transported to the nerve terminals. They are also present, at lower levels, in various other eukaryotic tissues. 14-3-3 proteins appear to play important roles in a variety of signal transduction pathways, including those involved in cell cycle regulation and cell survival. Because 14-3-3 proteins bind to specific phosphoserine-containing sequences they are likely to have an important role in signaling pathways mediated by serine/threonine protein kinases. Evidence indicates 14-3-3 is required for Raf 1 kinase activity and phosphorylation among many other functions.

Alternate Names

1433 eta, Protein AS1, YWHA1, YWHAH

Gene Symbol

YWHAH

Additional 14-3-3 eta Products

Product Documents for 14-3-3 eta Antibody (3G12) [DyLight 755]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for 14-3-3 eta Antibody (3G12) [DyLight 755]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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