4-Hydroxynonenal Antibody Best Seller

4‑Hydroxynonenal in Human Prostate Cancer Tissue.

4-Hydroxynonenal was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-4-Hydroxynonenal Monoclonal Antibody (Catalog # MAB3249) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Mouse 4-Hydroxynonenal by Immunocytochemistry/Immunofluorescence

Diapocynin inhibits the formation of 3-nitrotyrosine (3-NT) and 4-hydroxynonenal (4-HNE) in the substantia nigra (SN) of MPTP-treated mice. (A) Representative Western blots illustrating the expression of 3-NT in SN. (B) Bar graph showing mean Western blot 3-NT/ beta-actin ratios ± SEM in SN of 6 mice per group. (C) Double labeling of tyrosine hydroxylase (TH) and 3-NT in SN region of ventral midbrain. (D) Representative Western blots illustrating the expression of 4-HNE in SN. (E) Bar graph showing mean Western blot 4-HNE/ beta-actin ratios ± SEM in SN of 6 mice per group. (F) Double labeling of TH and 4-HNE in SN region of ventral midbrain. Images were captured at 60× magnification. The SN zone is outlined in white dots. Inset pictures demonstrated colocalization of TH and 3-NT/4-HNE. ***P <0.001 vs the control group; **P <0.01 vs the control group; *P <0.05 vs the MPTP group; #P <0.001 vs the MPTP group. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23092448), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse 4-Hydroxynonenal by Western Blot

Diapocynin inhibits the formation of 3-nitrotyrosine (3-NT) and 4-hydroxynonenal (4-HNE) in the substantia nigra (SN) of MPTP-treated mice. (A) Representative Western blots illustrating the expression of 3-NT in SN. (B) Bar graph showing mean Western blot 3-NT/ beta-actin ratios ± SEM in SN of 6 mice per group. (C) Double labeling of tyrosine hydroxylase (TH) and 3-NT in SN region of ventral midbrain. (D) Representative Western blots illustrating the expression of 4-HNE in SN. (E) Bar graph showing mean Western blot 4-HNE/ beta-actin ratios ± SEM in SN of 6 mice per group. (F) Double labeling of TH and 4-HNE in SN region of ventral midbrain. Images were captured at 60× magnification. The SN zone is outlined in white dots. Inset pictures demonstrated colocalization of TH and 3-NT/4-HNE. ***P <0.001 vs the control group; **P <0.01 vs the control group; *P <0.05 vs the MPTP group; #P <0.001 vs the MPTP group. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/23092448), licensed under a CC-BY license. Not internally tested by R&D Systems.