5-HT2A Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16043
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for 5-HT2A Antibody - Azide and BSA Free
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human 5-HT2A (NP_000612.1). MDILCEENTSLSSTTNSLMQLNDDTRLYSNDFNSGEANTSDAFNWTVDSENRTNLSCEGCLSPSCLSLLHLQEKNWSALLTAVVIILTIAGNILVIMAVS
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for 5-HT2A Antibody - Azide and BSA Free
Western Blot: 5-HT2A AntibodyAzide and BSA Free [NBP3-16043]
Western Blot: 5-HT2A Antibody [NBP3-16043] - Analysis of extracts of various cell lines, using 5-HT2A antibody (NBP3-16043) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.Immunocytochemistry/ Immunofluorescence: 5-HT2A Antibody - Azide and BSA Free [NBP3-16043]
Immunocytochemistry/Immunofluorescence: 5-HT2A Antibody [NBP3-16043] - Immunofluorescence analysis of SH-SY5Y cells using 5-HT2A Rabbit pAb (NBP3-16043) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.Immunocytochemistry/ Immunofluorescence: 5-HT2A Antibody - Azide and BSA Free [NBP3-16043]
Immunocytochemistry/Immunofluorescence: 5-HT2A Antibody [NBP3-16043] - Immunofluorescence analysis of Neuro-2a cells using 5-HT2A Rabbit pAb (NBP3-16043) at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.Applications for 5-HT2A Antibody - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 50% glycerol, pH7.3
Format
Azide and BSA Free
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: 5-HT2A
Long Name
5-Hydroxytryptamine Receptor 2A
Alternate Names
5HT2A, HTR2A
Gene Symbol
HTR2A
Additional 5-HT2A Products
Product Documents for 5-HT2A Antibody - Azide and BSA Free
Product Specific Notices for 5-HT2A Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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