5-HT4 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78403

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.09 mg/ml

View all 5-HT4 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal portion of the human 5HT4 Receptor protein (between residues 350-388) [UniProt Q13639, isoform 1]

Localization

Cell membrane; Multi-pass membrane protein. Endosome. Note: Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for 5-HT4 Antibody - BSA Free

Western Blot: 5-HT4 AntibodyBSA Free [NBP1-78403]

Western Blot: 5-HT4 AntibodyBSA Free [NBP1-78403]

Western Blot: 5-HT4 Antibody [NBP1-78403] - Western Blot Image of anti-5HT4 Receptor. Total protein from human liver tissue was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-LYVE1 in 1% milk/TBST. Reactivity was detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/ Immunofluorescence: 5-HT4 Antibody - BSA Free [NBP1-78403]

Immunocytochemistry/ Immunofluorescence: 5-HT4 Antibody - BSA Free [NBP1-78403]

Immunocytochemistry/Immunofluorescence: 5-HT4 Antibody [NBP1-78403] - Staining of 5HT4 receptor in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).

Applications for 5-HT4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

2 ug/ml
Application Notes
This 5HT4 Receptor antibody is useful for Immunocytochemistry/Immunofluorescence where cytoplasmic staining is observed in HeLa cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.09 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 5-HT4

The 5HT4 receptor (5-HT4R) is a member of the serotonin receptors. In response to serotonin (5HT) these G protein coupled receptors cause an increase in cAMP production. It is a glycosylated, transmembrane protein found in the central and peripheral nervous system. Multiple transcripts with variant C-terminal sequnces have been described. The significance of SNP variants of 5HT4 receptors in mental diseases is a controversial topic (PMID:19328558, 19892407), while other SNP variants have been associated with asthma.( PMID:21382128). Additional research show that the early presence of these receptors in the dorsomedial nuclei of prenatal hypothalamus may indicate a crucial role in fetal development. (PMID:20538346)

Long Name

5-Hydroxytryptamine Receptor 4

Alternate Names

5HT4, HTR4

Entrez Gene IDs

3360 (Human)

Gene Symbol

HTR4

UniProt

Q13639 (Human)

Additional 5-HT4 Products

Product Documents for 5-HT4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for 5-HT4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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