Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (94%), Mouse (93%), Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Multiplex Immunofluorescence, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 45M4F9

Format

BSA Free

Concentration

1.0 mg/ml

View all 5'-Nucleotidase/CD73 Antibodies »

Product Specifications

Immunogen

A portion of amino acids 169-324 of human CD73 were used as the immunogen for the antibody.

Specificity

This product can detect Isoform 1 and Isoform of Human CD73.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for 5'-Nucleotidase/CD73 Antibody (45M4F9) - BSA Free

5'-Nucleotidase/CD73 Antibody (45M4F9) - BSA Free

Immersion fixed paraffin embedded sections of Human Lung cancer tissue

CD73 was detected in immersion fixed paraffin-embedded sections of human lung cancer using Mouse Anti-Human CD73 Monoclonal Antibody (Novus Catalog # NBP2-25237) at 1:200 at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder.
5'-Nucleotidase/CD73 Antibody (45M4F9) - BSA Free

Detection of CD73 in Human Cervical Cancer via Multiplex Immunofluorescence staining on COMET™

CD73 was detected in immersion fixed paraffin-embedded sections of human cervical cancer using Mouse Anti-Human CD73 Monoclonal Antibody (Novus Catalog # NBP2-25237) at 1:200 at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder.
5'-Nucleotidase/CD73 Antibody (45M4F9) - BSA Free

Detection of CD73 in Human Tonsil via Multiplex Immunofluorescence staining on COMET™

CD73 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD73 Monoclonal Antibody (Novus Catalog # NBP2-25237 ) at 1:200 at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder.
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Applications for 5'-Nucleotidase/CD73 Antibody (45M4F9) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

5 ug/ml

Multiplex Immunofluorescence

1:200

Western Blot

2-5 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: 5'-Nucleotidase/CD73

CD73 is a membrane-bound enzyme having a key role in extracellular ATP metabolism producing adenosine by dephosphorylating AMP. It is highly expressed on endothelial cells but a moderate expression is also observed on a subpopulation of lymphocytes, especially regulatory T cells. Although CD73 on the endothelial cells and lymphocytes are structurally similar, they are differently regulated. Triggering of lymphocyte CD73 with antibodies results in cleavage of CD73 and activation of LFA-1, leading to increased adhesion of lymphocytes to endothelium (Algars A et al., 2011). CD73 plays an important role in carcinogenesis, as adenosine promotes tumor progression and CD73-expressing cancer cell lines are more aggressive (Supernat A et al., 2012). Extracellular adenosine is a potent immunosuppressor that accumulates during tumor growth. Anti-CD73 monoclonal antibody therapy significantly delays primary 4T1.2 and E0771 tumor growth in immune-competent mice and significantly inhibits the development of spontaneous 4T1.2 lung metastases. Antibody therapy is essentially dependent on the induction of adaptive anti-tumor immune responses (Stagg J et al., 2010).

Alternate Names

5-NT, CD73, E5NT, eNT, NT5E, NTE

Gene Symbol

NT5E

UniProt

NP_001191742 (Human)

Additional 5'-Nucleotidase/CD73 Products

Product Documents for 5'-Nucleotidase/CD73 Antibody (45M4F9) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for 5'-Nucleotidase/CD73 Antibody (45M4F9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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