5'-Nucleotidase/CD73 Antibody (AD2) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80552
Clone AD2 was used by HLDA to establish CD designation.
![Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (AD2) - Azide and BSA Free [NBP2-80552]](https://resources.bio-techne.com/images/products/5-Nucleotidase-CD73-Antibody-AD2-Azide-and-BSA-Free-Flow-Cytometry-NBP2-80552-img0004.jpg "Flow Cytometry: 5'-Nucleotidase/CD73 Antibody (AD2) - Azide and BSA Free [NBP2-80552]")
![Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) - Azide and BSA Free [NBP2-80552]](https://resources.bio-techne.com/images/products/5-Nucleotidase-CD73-Antibody-AD2-Azide-and-BSA-Free-Flow-Cell-Surface-NBP2-80552-img0003.jpg "Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) - Azide and BSA Free [NBP2-80552]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, Flow (Cell Surface), Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # AD2
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for 5'-Nucleotidase/CD73 Antibody (AD2) - Azide and BSA Free
Immunogen
Human CD73 [UniProt# P21589]
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for 5'-Nucleotidase/CD73 Antibody (AD2) - Azide and BSA Free
Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) - Azide and BSA Free [NBP2-80552]
Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) - Azide and BSA Free [NBP2-80552] - A surface stain was performed on U87 MG cells with 5'-Nucleotidase/CD73 Antibody (AD2) antibody NBP2-48480 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by Dylight488-conjugated anti-mouse secondary antibody. Image from the standard format of this antibody.![Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) - Azide and BSA Free [NBP2-80552]](https://resources.bio-techne.com/images/products/5-Nucleotidase-CD73-Antibody-AD2-Azide-and-BSA-Free-Flow-Cell-Surface-NBP2-80552-img0001.jpg "Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) - Azide and BSA Free [NBP2-80552]")
Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) - Azide and BSA Free [NBP2-80552]
Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) - Azide and BSA Free [NBP2-80552] - A cell surface stain was performed on U87MG cells with NBP2-48480APC (blue) and a matched isotype control NBP2-27287APC (orange). Cells were incubated in an antibody dilution of 0.5 ug/mL for 20 minutes at room temperature. Both antibodies were directly conjugated to Allophycocyanin. Image using the Allophycocyanin form of this antibody.![Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) - Azide and BSA Free [NBP2-80552]](https://resources.bio-techne.com/images/products/5-Nucleotidase-CD73-Antibody-AD2-Azide-and-BSA-Free-Flow-Cell-Surface-NBP2-80552-img0002.jpg "Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) - Azide and BSA Free [NBP2-80552]")
Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) - Azide and BSA Free [NBP2-80552]
Flow (Cell Surface): 5'-Nucleotidase/CD73 Antibody (AD2) - Azide and BSA Free [NBP2-80552] - A surface stain was performed on human peripheral blood lymphocytes with CD73 (AD2) antibody NBP2-48480AF488 and a matched isotype control NBP2-27287AF488. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-25196AF647. Image from the standard format of this antibody.Applications for 5'-Nucleotidase/CD73 Antibody (AD2) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:500
Immunohistochemistry-Frozen
reported in scientific literature (PMID 1989648)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: 5'-Nucleotidase/CD73
Alternate Names
5-NT, CD73, E5NT, eNT, NT5E, NTE
Gene Symbol
NT5E
Additional 5'-Nucleotidase/CD73 Products
- All Products for 5'-Nucleotidase/CD73
- 5'-Nucleotidase/CD73 Activity Assays
- 5'-Nucleotidase/CD73 cDNA Clones
- 5'-Nucleotidase/CD73 ELISA Kits
- 5'-Nucleotidase/CD73 Lysates
- 5'-Nucleotidase/CD73 Primary Antibodies
- 5'-Nucleotidase/CD73 Proteins and Enzymes
- 5'-Nucleotidase/CD73 Small Molecules and Peptides
Product Documents for 5'-Nucleotidase/CD73 Antibody (AD2) - Azide and BSA Free
Product Specific Notices for 5'-Nucleotidase/CD73 Antibody (AD2) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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