A-RAF Antibody (OTI2G9) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70201
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # OTI2G9
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 145-365 of human ARAF (NP_001645) produced in E.coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for A-RAF Antibody (OTI2G9) - Azide and BSA Free
Western Blot: A-RAF Antibody (OTI2G9)Azide and BSA Free [NBP2-70201]
Western Blot: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARAF.Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201]
Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201]
Immunohistochemistry: A-RAF Antibody (OTI2G9) - Azide and BSA Free [NBP2-70201] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)Applications for A-RAF Antibody (OTI2G9) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:150
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: A-Raf
Long Name
v-Raf Murine Sarcoma 3611 Viral Oncogene Homolog
Alternate Names
ARaf, PKS, PKS2, RAFA1
Gene Symbol
ARAF
Additional A-Raf Products
Product Documents for A-RAF Antibody (OTI2G9) - Azide and BSA Free
Product Specific Notices for A-RAF Antibody (OTI2G9) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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