AAK1 Antibody (4B10)

Novus Biologicals, part of Bio-Techne | Catalog # H00022848-M02

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4B10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all AAK1 Antibodies »

Product Specifications

Immunogen

AAK1 (AAH02695, 315 a.a. ~ 415 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.LKKECPIPNVQNSPIPAKLPEPVKASEAAAKKTQPKARLTDPIPTTETSIAPRQRPKAGQTQPNPGILPIQPALTPRKRATVQPPPQAAGSSNQPGLLASV

Specificity

AAK1 - AP2 associated kinase 1 (4B10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for AAK1 Antibody (4B10)

Western Blot: AAK1 Antibody (4B10) [H00022848-M02]

Western Blot: AAK1 Antibody (4B10) [H00022848-M02]

Western Blot: AAK1 Antibody (4B10) [H00022848-M02] - Analysis of AAK1 expression in transfected 293T cell line by AAK1 monoclonal antibody (M02), clone 4B10.Lane 1: AAK1 transfected lysate(52 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: AAK1 Antibody (4B10) [H00022848-M02]

Immunocytochemistry/ Immunofluorescence: AAK1 Antibody (4B10) [H00022848-M02]

Immunocytochemistry/Immunofluorescence: AAK1 Antibody (4B10) [H00022848-M02] - Analysis of monoclonal antibody to AAK1 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: AAK1 Antibody (4B10) [H00022848-M02] - Detection limit for recombinant GST tagged AAK1 is 0.1 ng/ml as a capture antibody.

Applications for AAK1 Antibody (4B10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for Western Blot. Has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: AAK1

Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. This gene encodes a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Long Name

AP2-associated Protein Kinase 1

Alternate Names

Adaptor-associated kinase 1, AP2 associated kinase 1, AP2-associated protein kinase 1, DKFZp686F03202, DKFZp686K16132, EC 2.7.11.1, FLJ23712, FLJ25931, FLJ31060, FLJ45252, KIAA1048FLJ42882, MGC138170, MGC164568, MGC164570

Entrez Gene IDs

22848 (Human)

Gene Symbol

AAK1

UniProt

AAH02695 (Human)

Additional AAK1 Products

Product Documents for AAK1 Antibody (4B10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AAK1 Antibody (4B10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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