AAK1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77335

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all AAK1 Antibodies »

Product Summary for AAK1 Antibody - BSA Free

Immunogen

Antibody was raised against a 20 amino acid synthetic peptide near the amino terminus of the human Aak1. The immunogen is located within amino acids 190 - 240 of Aak1. Amino Acid Squence: TNKFQNPQTEGVNAVEDEIK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AAK1 Antibody - BSA Free

Western Blot: AAK1 AntibodyBSA Free [NBP1-77335]

Western Blot: AAK1 AntibodyBSA Free [NBP1-77335]

Western Blot: AAK1 Antibody [NBP1-77335] - A-20 lysate with Aak1 antibody at (A) 1 and (B) 2 ug/ml.
Immunocytochemistry/ Immunofluorescence: AAK1 Antibody - BSA Free [NBP1-77335]

Immunocytochemistry/ Immunofluorescence: AAK1 Antibody - BSA Free [NBP1-77335]

Immunocytochemistry/Immunofluorescence: AAK1 Antibody [NBP1-77335] - A-20 cells with AAK1 antibody at 5 ug/ml.
Immunohistochemistry: AAK1 Antibody - BSA Free [NBP1-77335]

Immunohistochemistry: AAK1 Antibody - BSA Free [NBP1-77335]

Immunohistochemistry: AAK1 Antibody [NBP1-77335] - Immunofluorescence of aak1 in human brain tissue with aak1 antibody at 20 ug/mL.

Applications for AAK1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

5-20 ug/ml

Western Blot

1-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AAK1

AP2-associated protein kinase 1 (Aak1) is a member of the Ark1/Prk1 subfamily of Ser/Thr protein kinases that are thought to regulate endocytosis by phosphorylating the accessory endocytic components. Aak1 interacts with and phosphorylates the mu2 subunit of the AP-2 complex, which promotes binding of the AP-2 to tyrosine based (Yxxphi) internalization motif-containing receptors and subsequent receptor endocytosis. At least two isoforms of Aak1 are known to exist; the longer isoform contains an extended carboxy-terminus that contains an additional clathrin-binding domain. Overexpression of this long isoform or Aak1 depletion by RNA interference impairs transferrin recycling from the early/sorting endosome, suggesting that Aak1 functions at multiple steps of the endosomal pathway by regulating transferrin internalization and its recycling back to the plasma membrane.

Long Name

AP2-associated Protein Kinase 1

Alternate Names

Adaptor-associated kinase 1, AP2 associated kinase 1, AP2-associated protein kinase 1, DKFZp686F03202, DKFZp686K16132, EC 2.7.11.1, FLJ23712, FLJ25931, FLJ31060, FLJ45252, KIAA1048FLJ42882, MGC138170, MGC164568, MGC164570

Gene Symbol

AAK1

UniProt

NP_055726 (Human)

Additional AAK1 Products

Product Documents for AAK1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AAK1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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