AAK1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84110

Novus Biologicals, part of Bio-Techne
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NBP1-84110
NBP1-84110-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all AAK1 Antibodies »

Product Summary for AAK1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LLNKDFAKLGEGKHPEKLGGSAESLIPGFQSTQGDAFATTSFSAGTAEKRKGGQTVDSGLPLLSVSDPFIPLQVPDAPEKLIEGLKSPDTSLLLPDLLPMTDPFGSTSDAVIGKVIISVSSVMHDMCA

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AAK1 Antibody

Immunohistochemistry-Paraffin: AAK1 Antibody [NBP1-84110]

Immunohistochemistry-Paraffin: AAK1 Antibody [NBP1-84110]

Immunohistochemistry-Paraffin: AAK1 Antibody [NBP1-84110] - Staining in human parathyroid gland and pancreas tissues using anti-AAK1 antibody. Corresponding AAK1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: AAK1 Antibody [NBP1-84110]

Immunohistochemistry-Paraffin: AAK1 Antibody [NBP1-84110]

Immunohistochemistry-Paraffin: AAK1 Antibody [NBP1-84110] - Staining of human colon, liver, lymph node and parathyroid gland using Anti-AAK1 antibody NBP1-84110 (A) shows similar protein distribution across tissues to independent antibody NBP1-84111 (B).
Immunocytochemistry/ Immunofluorescence: AAK1 Antibody [NBP1-84110]

Immunocytochemistry/ Immunofluorescence: AAK1 Antibody [NBP1-84110]

Immunocytochemistry/Immunofluorescence: AAK1 Antibody [NBP1-84110] - Staining of human cell line U-251 MG shows localization to plasma membrane.
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Applications for AAK1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AAK1

AAK1 (AP2-associated kinase 1) is a Ser/Thr protein kinase. The rat homolog was first identified in a screen of a rat brain cDNA library using a domain of the alpha subunit of the heterotetrameric AP2 complex as bait. The AP2 complex is one of two major coat proteins of endocytic vesicles that function in receptor-mediated endocytosis and the recycling of synaptic vesicles. AAK1 is responsible for phosphorylating the mu subunit of AP2 to promote clathrin-mediated endocytosis.

Long Name

AP2-associated Protein Kinase 1

Alternate Names

Adaptor-associated kinase 1, AP2 associated kinase 1, AP2-associated protein kinase 1, DKFZp686F03202, DKFZp686K16132, EC 2.7.11.1, FLJ23712, FLJ25931, FLJ31060, FLJ45252, KIAA1048FLJ42882, MGC138170, MGC164568, MGC164570

Entrez Gene IDs

22848 (Human)

Gene Symbol

AAK1

UniProt

Q2M2I8

Additional AAK1 Products

Product Documents for AAK1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for AAK1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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